STUDIES OF HETEROGENEITY OF STREPTOKINASES OF DIFFERENT ORIGIN

  • 1 January 1977
    • journal article
    • research article
    • Vol. 238  (3) , 336-349
Abstract
Streptokinases of 6 different strains of group A streptococci (Streptococcus pyogenes) and of 1 strain each of streptococcal groups C and G were isolated from supernatants of the cultures. The purified or partial purified streptokinases were characterized by electrophoresis in agar-acrylamide gels, isoelectric focussing in acrylamide gel and by determinations of the MW by sodium dodecylsulfate acrylamide electrophoresis and gel filtration on Sephadex G-100. Also these streptokinases were compared serologically using human antistreptokinase-antiserum. All of the streptokinases examined in this study were different in their isoelectric points (5.05-6.5) and they showed serological cross-reactions. The MW are not significantly different and were about 45,000 daltons.