AZOTOBACTER CHROOCOCCUM AND ITS RELATIONSHIP TO ACCESSORY GROWTH FACTORS

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Abstract
The following accessory growth factors were tested in varying concs. and found to be unnecessary in synthetic media for the normal growth and metabolism of A. chroococcum: ascorbic acid, biotin, carotene, colchicine, ergo-sterol, filtrate factor, indole-3-acetic acid, indole butyric acid, indole-3-propionic acid, [gamma] (indole 3) n-butyric acid, inositol, nicotinamide, nicotinic acid, pantothenic acid, pico-line, pimelic acid, thiamin, riboflavin, and pyridoxin. Thiamin, riboflavin, and ascorbic acid stimulated slightly in some concs., but no evidence was obtained that any of these compounds are essential or materially increase A. chroococcum metabolism. A. chroococcum when grown in a synthetic medium free from accessory food factors synthesized biotin, inositol, nicotinic acid, pantothenic acid, pyridoxin, riboflavin, and thiamin. The quantity synthesized compares favorably with the quantities found in other plant . tissues including yeast. It is believed that these compounds enter into the composition of the bacterial enzymes, most of which are essential in carbohydrate metabolism.