Mutations in the structural genes of CHO cell histidyl-, valyl-, and leucyl-tRNA synthetases
- 1 May 1978
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 4 (3) , 299-311
- https://doi.org/10.1007/bf01542844
Abstract
Forty-three temperature-sensitive mutants were isolated in the CHO cell line by selecting for noncycling cells using [3H]TdR and cytosine arabinoside. Cell division was extremely temperature sensitive in eight of the mutants, and these were studied in more detail. In seven of these eight mutants, the in vitro specific activity of a single aminoacyl-tRNA synthetase was greatly reduced; four had reduced levels of histidyl-tRNA synthetase, two of valyl-tRNA synthetase, and one of leucyl-tRNA synthetase. Cell hybridization studies showed that the mutants formed three complementation groups. In six of the seven mutants the aminoacyl-tRNA synthetase which had reduced activity was also more thermolabile than the wild-type enzyme. The spontaneous reversion frequency was low for these mutants, and in most cases could be increased by treatment with a chemical mutagen. The isolation of the valyl-tRNA synthetase mutant reported here brings to eight the number of different aminoacyl-tRNA synthetase mutants isolated in the CHO cell line.Keywords
This publication has 10 references indexed in Scilit:
- CHO cell mutants for arginyl-, asparagyl-, glutaminyl-, histidyl- and methionyl-transfer RNA synthetases: Identification and initial characterizationCell, 1977
- Temperature-Sensitive Mutations in Animal CellsAdvances in Cancer Research, 1977
- Selection of temperature-sensitive CHL asparagyl-tRNA synthetase mutants using the toxic lysine analog, S-2-aminoethyl-L-cysteineCell, 1976
- Mutants of the Chinese hamster ovary cell line requiring alanine and glutamateSomatic Cell and Molecular Genetics, 1976
- A leukaemic cell mutant with a thermolabile alanyl-transfer RNA synthetaseNature, 1975
- Temperature-sensitive cell cycle mutants: a chinese hamster cell line with a reversible block in cytokinesisCell, 1975
- Selection by [3H] amino acids of CHO-cell mutants with altered leucyl- and asparagyl-transfer RNA synthetasesSomatic Cell and Molecular Genetics, 1975
- A mammalian somatic ?cell cycle? mutant defective in G1Journal of Cellular Physiology, 1974
- Ouabain-resistant mutants of mouse and hamster cells in cultureCell, 1974
- Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.Proceedings of the National Academy of Sciences, 1968