An Improved Method for the Preparation of Chlorophyll by Means of Column Chromatography with Sepharose CL-6B

Abstract
An improved method for the preparation of chlorophyll a and chlorophyll b from fresh spinach leaves by column chromatography with Sepharose CL-6B was developed. A good separation of the green pigments was attained on the column with a mixed solvent program of 2, 3, 10 and 20% 2-propanol in hexane.