Responses of Epithelial-like Cells in Tissue Culture to Implant Materials

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Abstract
The toxicity of various implant materials was tested on human amnion cells. The cells were seeded into Leighton tubes containing 1 ml of Eagles basic medium to which 8% calf serum and antibiotics was added. Pieces of test material measuring 4x4x1 mm were thoroughly cleaned ultrasonically, rinsed in distilled water, dried and sterilized in sealed polyethylene bags by UV light prior to being placed in the seeded Leighton tubes under sterile conditions. A 9x22 mm coverslip was then positioned over the implant material to prevent movement or floating and all tubes were incubated at 37 [degree]C until plaque formation and monolayering occurred. The medium was changed if necessary. The cultures were examined microscopically and the degree of toxicity of the material compared to control cell line cultures. All metal implant materials were handled with teflon coated forceps to prevent metallic contamination. Metals to acrylics, porcelain, ostamer, silicones and numerous epoxy resins were assayed. 5 materials presented uniformly good responses with little or no cyto-toxicity and good monolayering of cells, 9 moderate cytolysis and interruption of cell layer integrity and 7 severe necrosis of cells with no monolayering. Others were in intermediate categories. Results are compared to other investigations.

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