High-performance liquid-chromatographic analysis for tryptophan in serum.
- 1 November 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (11) , 1984-1988
- https://doi.org/10.1093/clinchem/23.11.1984
Abstract
Concentrations of total tryptophan were determined rapidly and sensitively in 50 microliter of serum by a reversed-phase partition version of high-performance liquid chromatography. For determination of total tryptophan, sample preparation requires only precipitation of the serum protein with trichloroacetic acid and removing excess trichloroacetic acid with a 1,1,2-trichlorotrifluoroethane(Freon)/tri-N-octylamine solution. Tryptophan in serum samples was detected by ultraviolet and fluorescence spectrometry. No interferences from the naturally occurring constituents of serum were observed. Elution time for tryptophan is 15 min, the limit of detection is 1 pmol.This publication has 11 references indexed in Scilit:
- Selective monitoring of polynuclear aromatic hydrocarbons by high pressure liquid chromatography with a variable wavelength detectorAnalytical Chemistry, 1976
- An automated fluorometric method for the measurement of tryptophan in plasmaJournal of Agricultural and Food Chemistry, 1976
- The influence of sex, age and breast cancer on tryptophan metabolismClinica Chimica Acta; International Journal of Clinical Chemistry, 1967
- Tryptophan in human plasmaClinica Chimica Acta; International Journal of Clinical Chemistry, 1963
- The binding of l-tryptophan in human plasma at 37 °CArchives of Biochemistry and Biophysics, 1961
- Effects of Amino Acid Feedings in Schizophrenic Patients Treated with IproniazidScience, 1961
- UNBOUND AMINO ACID CONCENTRATIONS IN PLASMA, ERYTHROCYTES, LEUKOCYTES AND URINE OF PATIENTS WITH LEUKEMIA*Journal of Clinical Investigation, 1960
- The Specific Binding of l-Tryptophan to Serum AlbuminJournal of Biological Chemistry, 1958
- Unbound Amino Acid Concentrations in Human Blood PlasmasJournal of Clinical Investigation, 1957
- THE FREE AMINO ACIDS OF HUMAN BLOOD PLASMAJournal of Biological Chemistry, 1954