Expression of human liver epoxide hydrolase in Saccharomyces pombe
- 1 May 1988
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 251 (3) , 931-933
- https://doi.org/10.1042/bj2510931
Abstract
Human liver microsomal epoxide hydrolase cDNA was inserted into the yeast expression vector pEVP11. The resulting recombinant plasmid was introduced into Saccharomyces pombe. The epoxide hydrolase protein and enzymic activity was subsequently expressed and identified in the 105,000 g pellet after centrifugal fractionation of homogenized yeast cells. This method will provide a useful source of human liver epoxide hydrolase, avoiding the problems of obtaining human tissue.This publication has 17 references indexed in Scilit:
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