Purification of Highly Radioactive Mouse Interferon Produced by Ehrlich Ascites Tumour Cells Induced by Sendai Virus

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Abstract
Mouse interferon (IFN) was produced to high titers after induction of Ehrlich ascites tumor cells with Sendai virus by using an improved procedure. The IFN molecules were labeled during their synthesis by the incorporation of [3H]leucine and [3H]lysine. Electrophoretically homogeneous labeled IFN with a MW of 34,000 was obtained after a 2-step purification procedure using poly(I)-agarose and octyl-Sepharose column chromatography followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The specific radioactivity of this IFN was .apprx. 10 counts/min per IFN unit.