Phosphorylations and dephosphorylations in yeast and their inhibition by various agents

Publisher Website
Google Scholar
Abstract
Rates of esterification of inorganic phosphate within the yeast cell during the first minute after addition of glucose were determined, in nitrogen and in air. Levels of inorganic phosphate in the stationary phases of fermentation and respiration also were determined. The rate of hydrolysis of the esters formed, when further esterification is inhibited with urethane, were measured. The effect of various poisons on the rates of aerobic and anaerobic phosphorylation and on the minimum levels of inorganic phosphate in yeast were measured. An attempt is made to relate these effects to the effect of the same substances on glucose catabolism and polysaccharide synthesis. Various discrepancies are pointed out. The hypothesis of Lynen (1941) and of Lynen and Koenigsberger (1951) that the Pasteur effect is related to the lower inorganic phosphate level obtaining aerobically appears to be untenable, for propionitrile, which inhibits the Pasteur effect, has no effect on phosphorylation phenomena.

This publication has 3 references indexed in Scilit: