CYTOTOXIC EFFECTS OF A LEUKOCIDIN FROM STAPHYLOCOCCUS-AUREUS

  • 1 January 1975
    • journal article
    • research article
    • Vol. 233  (2) , 147-152
Abstract
In an attempt ot improve the assay of leukocidin from S. aureus a combined vital fluorochrome staining procedure with acridine orange and ethidium bromide was developed. It was more suitable for the demonstration of leukocidic effects on [cattle and sheep] leukocytes than phase contrast microscopy. Damaged leukocytes fluoresced distinctly red and undamaged leukocytes, green. With the fluorochrome-method leukocidic changes also became evident in some lymphocytes. These were not clearly demonstrated by phase contrast microscopy.