Hydrogen Uptake and Exchange by Pea Root Nodules

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Abstract
Hydrogenase activity in pea root nodules was studied by following gas exchanges of hydrogen and deuterium. It was found that the nodules did not evolve hydrogen but that hydrogen was taken up when it was provided in the gas mixture. When increasing partial pressures of deuterium were supplied, hydrogen was evolved at a rate which increased as the pressure of deuterium increased. Deuterium was taken up at the same time as this hydrogen was evolved. Hydrogen evolution in the presence of deuterium was inhibited by nitrogen, while the uptake of deuterium remained unaffected. It was concluded that pea root nodules have at least two separate hydrogenase system that are working in opposite directions and must thus be situated in sites of different oxidation-reduction potentials within the nodule.

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