Cloning and Sequencing of the Ribosomal RNA Genes in Maize: The 17S Region
- 1 February 1984
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 3 (1) , 31-40
- https://doi.org/10.1089/dna.1.1984.3.31
Abstract
The maize genes for the 17S, 5.8S, and 26S ribosomal RNAs (rRNAs) are located on chromosome 6 and consist of 9-kb sequences repeated about 5,000–10,000 times per 2C. One of these sequences was isolated from a λ library containing maize Eco RI genomic segments. The sequence of the small-subunit (17S) RNA was determined using the M13 shotgun-dideoxy sequencing approach. The maize sequence was compared with nuclear rRNAs from yeast, Xenopus, and rat. Using these sequences, it is possible to identify tentatively the start and the end points of the sequence of the maize nuclear small subunit rRNA. This RNA has a length of 1809 nucleotides. The alignment of all four sequences for maximal homology allows us to identify regions within the rRNA that have been conserved during eukaryotic evolution.This publication has 35 references indexed in Scilit:
- Apple II Computer Software for DNA and Protein Sequence DataDNA, 1983
- Cloning and sequence analysis reveal structural variation among related zein genes in maizeCell, 1982
- Construction and characterization of new cloning vehicles VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplicationGene, 1982
- Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequenceNature, 1981
- Secondary Structure of 16 S Ribosomal RNAScience, 1981
- Sequence and organization of the human mitochondrial genomeNature, 1981
- A versatile primer for DNA sequencing in the M13mp2 cloning systemGene, 1980
- The primary structure of 16S rDNA from Zea mays chloroplast is homologous to E. coli 16S rRNANature, 1980
- Escherichia coli lac operator mRNA affects translation initiation of β-galactosidase mRNANature, 1979
- Kinetoplast RNA of Leishmania tarentolaeCell, 1978