Solid-phase Hemadsorption

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Abstract
A solid-phase hemadsorption (SPHA) technique for rapid detection of T. pallidum-specific Ig[immunoglobulin]M antibodies [Ab] is described. Demonstration of these Ig permits differentiation between recent and long-lasting infection and facilitates the decision as to whether or not a patient should receive therapy for syphilis. Errors that occur in the fluorescent treponemal Ab-absorbed test with IgM conjugate (IgM-FTA-Ab test) because of high titers of T. pallidum-specific IgG (competitive inhibition), rheumatoid factors or 7S IgM do not occur with the SPHA technique. This test is easy to perform; it requires only microtiter plates coated with antisera to human IgM in addition to the standard T. pallidum hemagglutination reagents. As compared with the Venereal Disease Research Laboratory test, the SPHA test has the advantage of being specific for T. pallidum; IgM Ab can be demonstrated earlier after infection and disappear sooner after adequate treatment of syphilis.

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