Characterization of placental luteinizing hormone-relasing factor-like material

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Abstract
High pressure liquid chromatography (HPLC) and radioimmunoassay [RIA] were employed to characterize luteinizing hormone-releasing factor (LRF)-life material in the human placenta. Methanol extracts of the placenta were washed with acetic acid and chloroform, further purified on coarse octadecylsilane columns, fractionated on HPLC, and tested by RIA. In HPLC, placental LRF had the same retention time as synthetic LRF, and such fractions gave an inhibition curve which was parallel to that of synthetic LRF in RIA. Human placental LRF is similar or identical to LRF in the CNS.