Specificity control in solid-phase enzyme immunoassay for HBsAg by one-step in situ blocking with human anti-HBs.

Abstract
The specificity of 2 human antisera (anti-HBs) to hepatitis B surface antigen (HBsAg) of differing subspecificities as neutralizing antibody in the solid phase enzyme immunoassay for HBsAg was determined. Anti-HBs of subspecificity ay was slightly more effective in neutralizing subtype ay than ad and vice versa. However, both anti-HBs could be used equally well for neutralization of both subtypes.