Humoral Antibody, Plaque, and Rosette Formation in Mice treated with Thiamphenicol and Chloramphenicol

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Abstract
Humoral antibody (anti-sheep haemagglutinin and haemolysin) production, and in vitro plaque and rosette formation by spleen cells in mice, assayed 4 days after a single intraperitoneal injection of sheep red blood cells (SRBC), were inhibited by thiamphenicol or chloramphenicol injected plaques subcutaneously in single daily doses of 150 and 750 mg/kg respectively, for periods of 4–11 days beginning from 4 h to 11 days before the SRBC injection and ending 1 day before or 4 days after it. The total number of spleen cells in the treated mice was less or not reduced. These results are interpreted as favouring the hypothesis which ascribes the immunosuppressive properties of chloramphenicol and thiamphenicol to their anti-proliferative action on cells with an increased mitotic activity (as that which precedes antibody production) rather than to their interfering with polyribosome formation and hence γ-globulin synthesis in the antibody-forming cells.

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