EVIDENCE FOR THE EXISTENCE OF A SINGLE-STRANDED STAGE OF T2 BACTERIOPHAGE DURING REPLICATION

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Abstract
T2r+ and T2r+ Escherichia coli B complexes at different times in the latent period have been irradiated with monochromatic UV light at different wavelengths. The action spectrum for the phage alone shows a minimum at about 2350 A. The shape of the action spectrum remains constant until 3 minutes after injection, at which time the minimum, shifts to 2400 A. The minimum is again seen at 2350 A eleven minutes after injection. These shifts in the action spectrum are interpreted as a change in viral DNA from a double-strandedto a single-stranded configuration at three minutes and back to a double-stranded state by eleven minutes.