THE SUBCUTANEOUS ASSAY OF CORTICOTROPHIN A

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Abstract
Two methods, the Sayers test and the U. S. P. XV subcutaneous assay were compared with regard to the potency ratio of corticotropin A as compared with crude corticotropin and the slope of the dose-response curve. The results indicate that the potency ratio of pure corticotropin A to crude corticotropin was about 3 times larger in the subcutaneous assay than in the Sayers test. The subcutaneous assay had the same accuracy as the Sayers test and yielded the same results whether saline or gelatin was used as the solvent. The slope of the dose-response curve of corticotropin A was steeper than that of the U.S.P. corticotropin reference standard in the subcutaneous assay, when both were dissolved in gelatin.
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