Intracellular Localization of Tyrosine Hydroxylase Immunoreactivity in the Rat Adrenal Chromaffin and Pheochromocytoma Cells
- 1 January 1989
- journal article
- research article
- Published by S. Karger AG in Cells Tissues Organs
- Vol. 136 (1) , 1-8
- https://doi.org/10.1159/000146788
Abstract
The localization of tyrosine hydroxylase (TH) immunoreactivity in rat adrenal chromaffin and pheochromocytoma (PC12) cells was investigated by immunoelectron microscopy using monoclonal and polyclonal anti-sera against TH purified from rat adrenal medulla. Strong TH immunoreactivity was found uniformly in the granules of the adrenaline cells; the immunoreactivity was visible mainly within the periphery, but not in the clear space of the granules of the noradrenaline cells. In the PC12 cells, strongTH immunoreactivity was also observed uniformly in the granules. In addition, TH immunoreactivity was seen in the cytoplasm, the ribosomes attached to the endoplasmic reticulum and the free ribosomes of both the rat adrenal chromaffin and PC12 cells. These results suggest thatTH may be localized in the granules, cytoplasm and ribosomes of rat adrenal chromaffin and PC12 cells.Keywords
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