A Century of Enzyme Kinetics: Reliability of the K M and v max Estimates
- 1 March 2003
- journal article
- Published by Taylor & Francis
- Vol. 8 (2-3) , 169-187
- https://doi.org/10.1080/08948550302453
Abstract
The application of the quasi-steady-state approximation (QSSA) in biochemical kinetics allows the reduction of a complex biochemical system with an initial fast transient into a simpler system. The simplified system yields insights into the behavior of the biochemical reaction, and analytical approximations can be obtained to determine its kinetic parameters. However, this process can lead to inaccuracies due to the inappropriate application of the QSSA. Here we present a number of approximate solutions and determine in which regions of the initial enzyme and substrate concentration parameter space they are valid. In particular, this illustrates that experimentalists must be careful to use the correct approximation appropriate to the initial conditions within the parameter spaceKeywords
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