Effect of Methionine and Sulfate on the Metabolism of Cephalosporium acremonium.
- 1 December 1968
- journal article
- Vol. 16 (12) , 1913-8
Abstract
The metabolism of Cephalosporium acremonium grown in a complex medium supplemented with DL-methionine or inorganic sulfate was studied. More growth occurred in a sulfate medium than in a methionine medium. Methionine-grown cells had an increased rate of respiration, a higher rate of catabolism with acetate and glucose as substrate, and higher specific activities of certain respiratory enzymes than sulfate-grown cells. Labeled acetate and glucose were assimilated at a faster rate by methionine-grown cells than sulfate-grown cells. Taurine, cystathionine, and small quantities of four acidic compounds were present in the amino acid pool of methionine-grown cells, but they were not detected in the pool of sulfate-grown cells. The differences in metabolic activity of sulfate and methionine-grown cells are discussed in regard to cephalosporin C synthesis.This publication has 10 references indexed in Scilit:
- Biosynthesis of penicillin N and cephalosporin C. Antibiotic production and other features of the metabolism of a Cephalosporium sp.Biochemical Journal, 1967
- Effect of metal ions on hog liver esteraseArchives of Biochemistry and Biophysics, 1965
- Enzymatic Synthesis and Cleavage of Cystathionine in Fungi and BacteriaJournal of Biological Chemistry, 1965
- Studies of Methionine Synthesis in Coprinus lagopusJournal of General Microbiology, 1964
- Amino acid composition of fungi during development in submerged cultureBiochemical Journal, 1964
- Biosynthesis of cephalosporin C from amino acidsBiochemical Journal, 1963
- The cleavage and synthesis of cystathionine in wild type and mutant strains of Neurospora crassaBiochimica et Biophysica Acta, 1957
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acidsBiochimica et Biophysica Acta, 1950
- Quantitative Determination of Carbohydrates With Dreywood's Anthrone ReagentScience, 1948