MONOCLONAL-ANTIBODIES NEUTRALIZING THE ANTIVIRAL AND THE ANTIPROLIFERATIVE ACTIVITIES OF HUMAN INTERFERON-GAMMA
- 1 July 1988
- journal article
- research article
- Vol. 32 (4) , 334-338
Abstract
Spleen cells from BALB/c mice immunized with human leukocyte interferon gamma (HuIFN-gamma) were fused with mouse NSO myeloma cells. Nine hybridoma lines were found secreting monoclonal antibodies (MoAb) which were able to neutralize the antiviral activity of HuIFN-gamma. All nine MoAb inhibited also the antiproliferative activity of HuIFN-gamma. The ability of tested MoAb to inhibit both the antiviral and antiproliferative activities of HuIFN-gamma supports the suggestion that a common domain on HuIFN-gamma molecule might be responsible for both biological activities. However, ELISA and/or RIA proved unsuitable for detection of these specific MoAb.This publication has 7 references indexed in Scilit:
- DISTINCT FUNCTIONAL DOMAINS ON THE RECOMBINANT HUMAN IMMUNE INTERFERON MOLECULE1986
- Purification of Natural Human Interferon-Gamma by Antibody Affinity Chromatography: Analysis of Constituent Protein Species in the Dimers1The Journal of Biochemistry, 1986
- A High-Affinity Monoclonal Antibody (GIF-1) to Human Gamma-interferon: Neutralization of Interferon Mediated Inhibition of Retrovirus Production and 2’-5’ (A) Synthetase InductionPathobiology, 1986
- Human Interferon-γ Enhances the Expression of Class I and Class II Major Histocompatibility Complex Products in Neoplastic Cells More Effectively than Interferon-α and Interferon-βInfection and Immunity, 1983
- Mouse Monoclonal Antibody with Specificity for Human Interferon GammaHybridoma, 1983
- Differential efficacies of human type I and type II interferons as antiviral and antiproliferative agents.Proceedings of the National Academy of Sciences, 1980
- Inhibition of Murine Osteogenic Sarcomas by Treatment With Type I or Type II Interferon2JNCI Journal of the National Cancer Institute, 1978