MIGRATION OF HUMAN LYMPHOCYTES .1. MODEL USING MOUSE AS HOST

  • 1 January 1978
    • journal article
    • research article
    • Vol. 35  (5) , 695-705
Abstract
The distribution of radioactivity after i.v. injection of 51Cr-labeled human lymphocytes was examined in normal mice, irradiated mice, mice treated with anti-platelet antiserum and in mice treated with colloidal carbon. Pre-treatment with carbon and anti-platelet antiserum appeared to protect the human lymphocytes from uptake by the host''s RES. Comparison of tissue radioactivity in carbon-treated mice after the injection of viable human lymphocytes with that found after the injection of dead cells and soluble or insoluble cell debris showed that radioactivity recovered in the spleen and lymph nodes was primarily due to the migration of viable lymphocytes into these tissues. The measurement of radioactivity in lymph nodes of carbon-treated mice after the injection of 51Cr-labeled human lymphocytes can be used as a model of these lymphocytes'' ability to migrate into the lymph nodes during recirculation and to study factors influencing this migration.