An enzyme-linked immunosorbent assay for the measurement of thyroglobulin in human serum using mouse monoclonal antibodies

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Abstract
A robust, rapid, sensitive and specific enzyme-linked immunosorbent assay (ELISA), using an in-house immunoglobulin-A-sub-class mouse monoclonal human-thyroglobulin antibody (WNSM2), with a sensitivity of 1·51 pmol/l has been established for the measurement of thyroglobulin in serum. Standard curves in varying dilutions of human serum were similar to standard curves obtained in serum-free medium, thus demonstrating no significant cross-reactivity with any serum proteins other than thyroglobulin. Levels of serum thyroglobulin detected by ELISA correlated significantly (r = 0·93, P < 0·001) with those from a standardized and well-characterized radioimmunoassay. The coefficients of variation within and between ELISA assays were 3·9 and 7·1% respectively. Thyroglobulin was detectable in 87% of 54 normal subjects who had no history of thyroid or autoimmune disease, the mean (±s.d.) for this group being 15 ± 6·6 pmol/l with a range of 1·51–53 pmol/l. Using this assay, levels of thyroglobulin were shown to be significantly (P < 0·002) increased in patients with untreated hyperthyroid Graves' disease compared with normal subjects. J. Endocr. (1985) 107, 383–387