Bidirectional replication of the mini-ColE1 plasmid pVH51
Open Access
- 1 April 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 138 (1) , 257-260
- https://doi.org/10.1128/jb.138.1.257-260.1979
Abstract
Replicating molecules of the min-ColE1 plasmid pVH51 have been examined by electron microscopy after cleavage with the restriction endonuclease EcoRI. Replication apparently starts at a unique site indistinguishable from the origin of replication used by the parental plasmid ColE1. In contrast to ColE1, the structure of the majority of the replication intermediates was consistent with a bidirectional mode of replication. A minor portion of the molecules appeared to replicate unidirectionally in either direction from the same origin.This publication has 12 references indexed in Scilit:
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