THE EFFECT OF ANAËROBIOSIS ON THE EGGS AND SPERM OF SEA URCHIN, STARFISH AND NEREIS AND FERTILIZATION UNDER ANAËROBIC CONDITIONS

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Abstract
The eggs of sea urchin and Nereis are insensitive to anaerobiosis for as long as 5 hrs., those of starfish are more sensitive to lack of 0. The sperm of sea urchin are very sensitive to anaerobiosis, those of starfish are less sensitive. The sperm of Nereis can be kept as long as 5 hrs. in anaerobic conditions without any injury of their fertilization power. Fertilization of Nereis eggs can be started when both eggs and sperm have been kept in anaerobiosis 5 hrs. The fertilization process is stopped with the formation of the fertilization cone. No nuclear changes are seen. Fertilization is started in sea urchin and starfish eggs when fresh sperm are used. The process stops with the formation of the fertilization membrane.

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