ENZYME-LINKED IMMUNOSORBENT-ASSAY IN THE SERODIAGNOSIS OF SYPHILIS

1 January 1982

journal article
research article

Vol. 29 (4) , 221-226

Abstract

Enzyme-linked immunosorbent assay [ELISA] using ultrasonic lysate of Treponema pallidum and T. reiteri as antigens was used for the detection of antisyphilitic antibodies in various stages of syphilis. The conjugate was goat antiserum to human IgG labeled with horseradish peroxidase. A comparison with results of the T. pallidum immobilization test, rapid plasma reagin test, Kolmer complement fixation reaction using cardiolipin and Reiter protein antigens showed that ELISA was more sensitive, but less specific, with T. pallidum antigen. Absorption of group specific treponemal antibodies was needed to make the method reliable.

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