FELINE LEUKEMIA VIRUS-INDUCED ERYTHROID APLASIA - INVITRO HEMATOPOIETIC CULTURE STUDIES

  • 1 January 1981
    • journal article
    • research article
    • Vol. 9  (10) , 990-1001
Abstract
Colony forming unit (CFU) assays were developed for feline granulocyte-macrophage (CFUGM), early erythroid (day 2 CFUE) and late erythroid (day 7 CFUE) colonies in methylcellulose medium. Feline CFUGM and both day 2 and day 7 CFUE were enhanced by feline macrophage conditioned medium and late CFUE often were intimately associated with macrophages. Kittens were inoculated with the Kawakami-Theilen (KT) strain of feline leukemia virus (FeLV) and sequential changes in marrow CFU determined. Erythroid aplasia, characterized by progressive non-regenerative anemia, lymphopenia and a profound decrease in early and late CFUE but not CFUGM was induced by 3-5 wk after FeLV-KT inoculation. The susceptibility of kittens to FeLV-induced erythroid aplasia was strongly age-related; neonatal kittens were most sensitive and substantial natural resistance developed by 4 wk of age. FeLV-KT infection induced a rapid and selective suppression of erythroid progenitor cells and represents a suitable model of experimentally-induced acquired erythroid aplasia.