Chromosome measurement and sorting by flow systems.
- 1 April 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (4) , 1231-1234
- https://doi.org/10.1073/pnas.72.4.1231
Abstract
A flow microfluorometer was used to measure metaphase chromosomes in suspension at rates up to 100,000 per min. Chromosomes from cells of the Chinese hamster M3-1 cell line were isolated, stained for DNA with the fluorescent dye ethidium bromide, and analyzed for DNA content. Nine distinct peaks were resolved that correspond well with independent chromosomal DNA measurements made with a high-resolution scanning cytophotometer. Chromosomes were sorted from each peak by an electronic cell sorter. Visual examination of each fraction indicated the purity of the sorted chromosomes. This novel technology allows separation of purified populations of individual chromosomes suitable for biochemical and biological characterizations.Keywords
This publication has 7 references indexed in Scilit:
- Microfluorometric Detection of Deoxyribonucleic Acid Replication in Human Metaphase ChromosomesProceedings of the National Academy of Sciences, 1973
- Do homologous chromosomes differ?Cytogenetic and Genome Research, 1973
- Zonal Fractionation of Mammalian Metaphase Chromosomes and Determination of Their DNA ContentPreparative Biochemistry, 1973
- Individualized human karyotyping through quantitative analysisComputers in Biology and Medicine, 1972
- The use of proteolytic enzymes for the mapping of structural rearrangements in the chromosomes of manChromosoma, 1972
- A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pHExperimental Cell Research, 1970
- Identification of human chromosomes by DNA-binding fluorescent agentsChromosoma, 1970