Radioimmunoassay and chemical ionization/mass spectrometry compared for plasma cortisol determination.

Open Access

1 January 1982

journal article
research article
Published by Oxford University Press (OUP) in Clinical Chemistry

Vol. 28 (1) , 174-177
https://doi.org/10.1093/clinchem/28.1.174

Abstract

We describe a method for determination of cortisol in plasma and urine, based on chemical ionization/mass spectrometry with deuterium-labeled cortisol as the internal standard. The within-run precision (CV) was 2.5-5.7%, the between-run precision 4.6%. Results by this method were compared with those by a radioimmunological method (RIANEN Cortisol, New England Nuclear) for 395 plasma samples. The latter method gave significantly higher (approx. 25%) cortisol values.

This publication has 1 reference indexed in Scilit:

Interlaboratory evaluation of four RIA kits for determination of plasma cortisol, with special reference to accuracy: influence of matrix in calibration standards.
Clinical Chemistry, 1980