Locked Nucleic Acid: High-Affinity Targeting of Complementary RNA for RNomics
- 1 January 2006
- book chapter
- Published by Springer Nature
- Vol. 173 (173) , 405-422
- https://doi.org/10.1007/3-540-27262-3_21
Abstract
Locked nucleic acid (LNA) is a nucleic acid analog containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA-mimicking sugar conformation. This conformational restriction is translated into unprecedented hybridization affinity towards complementary single-stranded RNA molecules. That makes fully modified LNAs, LNA/DNA mixmers, or LNA/RNA mixmers uniquely suited for mimicking RNA structures and for RNA targeting in vitro or in vivo. The focus of this chapter is on LNA antisense, LNA-modified DNAzymes (LNAzymes), LNA-modified small interfering (si)RNA (siLNA), LNA-enhanced expression profiling by real-time RT-PCR and detection and analysis of microRNAs by LNA-modified probes.Keywords
This publication has 69 references indexed in Scilit:
- Locked nucleic acid containing antisense oligonucleotides enhance inhibition of HIV‐1 genome dimerization and inhibit virus replicationFEBS Letters, 2004
- Gaining Target Access for DeoxyribozymesJournal of Molecular Biology, 2004
- An autonomous molecular computer for logical control of gene expressionNature, 2004
- MicroRNAsCell, 2004
- Asymmetry in the Assembly of the RNAi Enzyme ComplexCell, 2003
- RNA Interference in Mammalian Cells by Chemically-Modified RNABiochemistry, 2003
- MicroRNAs and Other Tiny Endogenous RNAs in C. elegansCurrent Biology, 2003
- A map of human genome sequence variation containing 1.42 million single nucleotide polymorphismsNature, 2001
- Initial sequencing and analysis of the human genomeNature, 2001
- Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysJournal of Molecular Endocrinology, 2000