Site directed mutants of human interleukin‐1α: A 1H‐NMR and receptor binding study
- 11 April 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 231 (1) , 135-138
- https://doi.org/10.1016/0014-5793(88)80717-8
Abstract
Mutant human interleukin-1α proteins were constructed by oligonucleotide directed mutagenesis. Six different mutants were tested for receptor binding activity and showed no alteration with respect to the wild-type protein. Analysis of these mutants by nuclear magnetic resonance spectroscopy confirmed the structural integrity of the mutant proteins and permitted the sequence specific assignment of the histidine and tryptophan residuesKeywords
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