A cellulase complex in culture filtrates of Penicillium citrinum
- 1 August 1976
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 22 (8) , 1153-1159
- https://doi.org/10.1139/m76-167
Abstract
During growth in a liquid medium that contained a single soluble or an insoluble cellulosic carbon source Penicillium citrinum released a complex of cellulase enzymes into the medium. A temperature of 30 °C was best for cellulase production. Presence of carbon-containing compounds, particularly glucose, inhibited cellulase activity. The enzyme complex was separated by gel filtration followed by ion-exchange chromatography into 11 components, 4 of high molecular weight and 7 of low molecular weight. One of the components (Bb) had the character of C1 cellulase enzyme. When the components were combined they released more reducing sugars from cullulosic substrates than when they were used singly.This publication has 4 references indexed in Scilit:
- Inhibition of CellulasesAnnual Review of Phytopathology, 1965
- Estimation of the molecular weights of proteins by Sephadex gel-filtrationBiochemical Journal, 1964
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- THE BIOLOGICAL DEGRADATION OF SOLUBLE CELLULOSE DERIVATIVES AND ITS RELATIONSHIP TO THE MECHANISM OF CELLULOSE HYDROLYSISJournal of Bacteriology, 1950