Characterization of bleomycin action on DNA.
- 1 January 1975
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 28 (7) , 537-542
- https://doi.org/10.7164/antibiotics.28.537
Abstract
Cleavage of SV40 DNA by bleomycin was assayed quantitatively in vitro in the presence of various polynucleotides. SV40 DNA was protected from bleomycin-induced cleavage by native or denatured DNA of other origins, poly dG-C.cntdot.poly dG-C, poly dA-T.cntdot.poly dA-T and poly dA-T (denatured) but not by tRNA of Escherichia coli, apurinic acid, poly dA, poly dT and various deoxyribooligonucleotides. Various bleomycins and their derivatives and various fragments of bleomycin were tested for possible activity in cleaving SV40 DNA and from the results some structure-activity relationships for the action of bleomycin to act on DNA were outlined. Actinomycin D stimulated bleomycin action while ethidium bromide inhibited it.This publication has 2 references indexed in Scilit:
- Complex formation between ethidium bromide and nucleic acidsPublished by Elsevier ,2009
- PHYSICAL AND CHEMICAL PROPERTIES OF THE APURINIC ACID OF CALF THYMUSJournal of Biological Chemistry, 1953