High performance liquid chromatography, thin layer chromatography and ultraviolet behaviour of flavone aglycones with unsubstituted B rings

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Abstract
Structural identification of flavonoid aglycones with unsubstituted B rings is rather difficult using the classical techniques, especially with respect to chemotaxonomic studies when only minute amounts of substances are available. In the present work, the thin layer chromatography and high performance liquid chromatography behaviour of these substances has been evaluated, and correlations between structural features, Rf values and elution order (tR) have been established. In addition, their ultraviolet spectra in methanol have proved useful to ascertain substitution patterns and location of free hydroxyls. Additional Information can be obtained by analysis of the permethylated derivatives obtained by methylation of the naturally occurring compounds.