Partial Purification of Urinary Erythropoietin.

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Abstract
The urinary erythropoietic stimulating factor has been partially purified by adsorption on kaolin followed by serial elution with ammonium acetate buffers of increasing pH and then with 1[image] NH4OH. Using this procedure, a 39% yield with a 230 fold purification of the active factor has been obtained. The purified preparation of the active factor produces metachromasia with toluidine blue, has an absorption peak at 280 m[mu] and migrates very slowly on paper strips in an electrophoretic apparatus. The similarity and differences between urinary and plasma erythropoietin are discussed.
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