The intracarotid 133Xenon injection method for measurement of cerebral blood flow (CBF) in rats: evaluation of the effect of bolus volume

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Abstract
Cerebral microcirculation is assumed not to be perturbed by the intraarterial injection used in determination of cerebral blood flow (CBF) with the intrarterial 133Xe technique (and in various assessments of blood brain barrier (BBB) permeability). The application of these techniques to the rat, where the injectate is large compared to normal blood flow, places this problem in focus. It has been claimed that since large intracarotid injections increased cerebral venous outflow, the CBF must also increase. This problem was studied in the rat by means of the intraarterial 133Xe injection technique, using a saline bolus injected in less than 1 s and found that CBF was unaltered at bolus volumes between 10 and 100 .mu.l. Furthermore, injection of 100-200 .mu.l saline during washout detection did not change the slope of the semilogarithmic wash-put curves. In spite of large intracarotid injections the CBF remained constant. The hemodilution produced by the saline bolus is not sufficient to influence CBF. Consequently, estimations of CBF yield valid results in the present rat preparation.

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