PCR‐SSO TYPING FOR DR4‐Dw SUBTYPES: APPLICATION TO UNRELATED BONE MARROW TRANSPLANT DONOR SELECTION
- 1 February 1991
- journal article
- Published by Wiley in International Journal of Immunogenetics
- Vol. 18 (1-2) , 97-104
- https://doi.org/10.1111/j.1744-313x.1991.tb00009.x
Abstract
Thirty-seven DR4-positive patient-unrelated bone marrow donor pairs previously DR/DQ restriction fragment length polymorphism (RFLP) typed and tested in mixed lymphocyte culture (MLC), have been DR4-Dw subtyped retrospectively using sequence specific oligonucleotide probes. We found that DR4-Dw subtyping substantially increased the accuracy of pre-MLC matching and could potentially accelerate donor searches by avoiding unnecessary MLC tests on Dw-mismatched donors.Keywords
This publication has 10 references indexed in Scilit:
- Shedding light on PCR contaminationNature, 1990
- DNA typing for class II HLA antigens with allele-specific or group-specific amplification. I. Typing for subsets of HLA-DR4Human Immunology, 1990
- AN ANALYSIS OF THE EFFECT OF HLA‐DP IN THE MIXED LYMPHOCYTE REACTIONInternational Journal of Immunogenetics, 1988
- HLA‐DRβIII and HLA‐DP induce comparable proliferation in primary mixed lymphocyte cultureTissue Antigens, 1988
- A simple salting out procedure for extracting DNA from human nucleated cellsNucleic Acids Research, 1988
- Both HLA-DR and HLA-DQ determinants contribute to HLA-Dw typingHuman Immunology, 1987
- Clonal analysis of HLA-DR and -DQ associated determinants: Their contribution to Dw specificitiesHuman Immunology, 1986
- HLA-Dw specificity assignments are independent of HLA-DQ, HLA-DR, and other class II specificities and define a biologically important segregant series which strongly activates a functionally distinct T cell subsetHuman Immunology, 1986
- Different functions and associations of HLA‐DR and HLA‐DQ(DC) antigens shown by serological, cellular and DNA assaysTissue Antigens, 1985
- First Level Testing of HLA-DR4-Associated New HLA-D Specificities: Dw13 (DB3), Dw14 (LD40), Dw15 (DYT), and DKT2Published by Springer Nature ,1984