The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica
- 1 January 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 185 (1) , 59-63
- https://doi.org/10.1042/bj1850059
Abstract
An endonuclease, AsuI, was isolated from extracts of A. subcylindrica on the basis of gel-electrophoretic analysis of digests of bacteriophage-.lambda. DNA with the partially purified extracts. The enzyme requires Mg2+, but no other cofactors. Endonuclease AsuI recognizes the interrupted tetranucleotide sequence: .**GRAPHIC**. and breaks the phosphodiester bonds indicated by the arrows to leave single-stranded trinucleotide projections at the 5''-termini of the DNA fragments.This publication has 16 references indexed in Scilit:
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