XenCSA: Cell Surface Antigens Related to the Major Glycoproteins (gp70) of Xenotropic Murine Leukemia Viruses

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Abstract
Antisera from rabbits immunized with rabbit corneal (SIRC) cells infected with xenotropic murine leukemia viruses (MuLV) were tested for their reactivity with MuLV and the surface of cells exogenously or endogenously infected with MuLV. The sera demonstrated class specificity for gp 70 determinants of intact xenotropic MuLV. Only molecules with characteristics of gp70 were precipitated out of radiolabeled antigens from the surface of cell lines exogenously infected with MuLV or from lymphocytes of normal mice. Cross-reactivity between virion envelope and cell surface antigens was demonstrated by absorption testing. The set of crossreactive antigens present on the envelopes of xenotropic MuLV and murine lymphocytes was termed XenCSA for xenotropic MuLV envelope-related cell surface antigen. XenCSA expression on thymocytes and spleen cells from 63 strains of inbred mice was assessed by immunofluorescence using flow microfluorometry. Lymphocytes from both tissues of all strains expressed XenCSA but to a highly variable extent. Based on mean fluorescence values, thymocytes and spleen cells from each strain were classified as being high or low for XenCSA. There was a high association for expression of XenCSA and GIX on thymocytes but no correlation with Fv-1, T1a, H-2, Pca-1, or GCSA. The level of XenCSA expressed on the cell surface was also independent of the amount of infectious xenotropic MuLV produced by those cells.

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