Application of Fluorescent Antibody to Exoerythrocytic Stages of Simian Malaria

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Abstract
Antibody to Plasmodium cynomolgi B strain was obtained by immunizing rhesus monkeys with a parasite-rich preparation of highly infected rhesus monkey blood that was treated with .5% saponin to lyse red cells. The antibody developed by the rhesus monkeys in complete Freund''s adjuvant was characterized by its ability to react with all stages of erythrocytic forms of P. cynomolgi after being labeled with fluorescein isothiocyanate in the direct fluorescent-antibody technique; poor reactivity with other parasites such as P. fieldi, P. knowlesi and P. vivax; precipitating antibody characteristics when tested by double diffusion in gel against sonicated parasites; and complement-fixing ability. The direct fluorescent-antibody technique on unfixed fresh-frozen tissue offers a simple approach to the study of exoerythrocytic stages of malaria.

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