Amperometric Detection of Glucose Released from Imune Lysis of Glusoce Loaded Liposome
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 15 (2) , 135-146
- https://doi.org/10.1080/00032718208064371
Abstract
Glucose released from complement mediated immunospecific damage to glucose loaded liposomes sensitized with lipid antigens was detected by an amperometric glucose enzyme electrode to devise a simple method for electrochemical immunoassay. This method involves the indirect determination of antibody and complement levels by measurement of the released glucose. The results obtained for rabbit and guinea pig serum were correlated with those obtained by spectrophotometry with excellent accuracy, indicating that the amperometric enzyme electrode can be another substitute for the spectrophotometric detection of glucose released from immune lysis of liposome loaded with glucose.This publication has 8 references indexed in Scilit:
- Thin-layer potentiometric analysis of lipid antigen-antibody reaction by tetrapentylammonium (TPA+) ion loaded liposomes and TPA+ ion selective electrodeAnalytical Chemistry, 1980
- Plate-shaped silver/silver halide reference electrode for determination of fluoride ion in microliter solution with fluoride ion selective electrodeAnalytical Chemistry, 1980
- LIPOSOME IMMUNOELECTRODEChemistry Letters, 1980
- Immunochemical determination of ganglioside GM2, by inhibition of complement-dependent liposome lysisJournal of Immunological Methods, 1977
- Immunological Properties of Model MembranesAnnual Review of Biochemistry, 1977
- Immune Lysis of Liposomes and Erythrocyte Ghosts Loaded with Spin LabelProceedings of the National Academy of Sciences, 1974
- Immune response of a liposomal model membrane.Proceedings of the National Academy of Sciences, 1968
- Effect of cholesterol incorporation on the sensitivity of liposomes to the polyene antibiotic, filipinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968