A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function
Top Cited Papers
- 29 September 2000
- journal article
- research article
- Published by Springer Nature in Nature Cell Biology
- Vol. 2 (11) , 784-791
- https://doi.org/10.1038/35041020
Abstract
Mutations in the LIS1 gene cause gross histological disorganization of the developing human brain, resulting in a brain surface that is almost smooth. Here we show that LIS1 protein co-immunoprecipitates with cytoplasmic dynein and dynactin, and localizes to the cell cortex and to mitotic kinetochores, which are known sites for binding of cytoplasmic dynein. Overexpression of LIS1 in cultured mammalian cells interferes with mitotic progression and leads to spindle misorientation. Injection of anti-LIS1 antibody interferes with attachment of chromosomes to the metaphase plate, and leads to chromosome loss. We conclude that LIS1 participates in a subset of dynein functions, and may regulate the division of neuronal progenitor cells in the developing brain.Keywords
This publication has 45 references indexed in Scilit:
- Nuclear MigrationThe Journal of cell biology, 2000
- Intracellular levels of the LIS1 protein correlate with clinical and neuroradiological findings in patients with classical lissencephalyAnnals of Neurology, 1999
- Graded reduction of Pafah1b1 (Lis1) activity results in neuronal migration defects and early embryonic lethalityNature Genetics, 1998
- Point Mutations and an Intragenic Deletion in LIS1, the Lissencephaly Causative Gene in Isolated Lissencephaly Sequence and Miller-Dieker SyndromeHuman Molecular Genetics, 1997
- A Revision of the Lissencephaly and Miller-Dieker Syndrome Critical Regions in Chromosome 17p13.3Human Molecular Genetics, 1997
- NudF, a nuclear migration gene in Aspergillus nidulans, is similar to the human LIS-1 gene required for neuronal migration.Molecular Biology of the Cell, 1995
- Cytoplasmic dynein and actin-related protein Arp1 are required for normal nuclear distribution in filamentous fungi.The Journal of cell biology, 1994
- Miller-Dieker lissencephaly gene encodes a subunit of brain platelet-activating factorNature, 1994
- Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans.Proceedings of the National Academy of Sciences, 1994
- Isolation of a Miller–Dicker lissencephaly gene containing G protein β-subunit-like repeatsNature, 1993