The Assessing of Acidophilia with Biebrich Scarlet, Ponceau De Xylidine and Woodstain Scarlet
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 54 (1) , 13-16
- https://doi.org/10.3109/10520297909110668
Abstract
The bonding of Biebrich scarlet at an alkaline pH was reportedly hydrophobic, as the isomers of Biebrich scarlet (ponceau de xylidine and woodstain scarlet) do not stain at that pH. If correct, this would negate the use of Biebrich scarlet as a measure of acidophilia. All 3 isomers stained similarly at an alkaline pH. Biebrich scarlet saturated with urea stained elastic fibers only faintly or not at all. The staining of other acidophilic structures (Paneth cell granules, sperm heads, etc.) was blocked. If only H bonding and not ionic bonding is blocked in an aqueous dye solution saturated with urea, then the exact mechanism of alkaline Biebrich scarlet staining is unknown.This publication has 4 references indexed in Scilit:
- Notes on TechnicStain Technology, 1963
- HISTOCHEMICALLY SELECTIVE ACIDOPHILIA OF BASIC NUCLEOPROTEINS IN CHROMATIN AND NUCLEOLI AT ALKALINE pHJournal of Histochemistry & Cytochemistry, 1962
- Histochemical Identification of Basic Proteins with Biebrich Scarlet at Alkaline pHStain Technology, 1961