Abstract
Synopsis: There were differences in yield, SO4‐S concentration, and total S uptake among competing species growing at different levels of available S. Yield response to S increased as the season advanced, but the concentration of total S and SO4‐S in the plants decreased. The SO4‐S concentration in each of the species where no S was applied was indicative of S deficiency. The SO4‐S concentration in subclover at flowering appeared to be the best single indicator of S status.

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