Non-radioactive labeling of RNA transcriptsin vitrowith the hapten digoxigenin (DIG); hybridization and ELISA-based detection
Open Access
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (19) , 5843-5851
- https://doi.org/10.1093/nar/18.19.5843
Abstract
We have developed a system for the enzymatic in vitro synthesis of non-radioactively labeled RNA which is derivatized with the hapten digoxigenin (DIG). The labeling reaction as well as the conditions for hybridization and detection of hybrids by an antibody-conjugate and a coupled colour reaction were analyzed and adapted for high sensitivity and low background. In addition, data on the performance and sensitivity of digoxigenin-labeled RNA probes in Southern and Northern blots are presented.This publication has 29 references indexed in Scilit:
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