Isoelectric Focusing of PGM1(E.C.2.7.5.1) on Agarose: Application to Cases of Disputed Parentage
Open Access
- 1 May 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 75 (5) , 708-711
- https://doi.org/10.1093/ajcp/75.5.708
Abstract
A method for isoelectric focusing of phosphoglucomutase (PGM1) using agarose was found to have significant advantages over published acrylamide technics. Gene frequencies determined on samples from 1,780 Minnesota Caucasians were PGM11+ = 0.6118, PGM11- = 0.1441, PGM12+ = 0.1910, PGM12- = 0.0531. The use of the isoelectric focusing technics in 700 cases of disputed parentage resulted in an exclusion rate slightly in excess of the calculated probability of exclusion (P value) of 27%. In addition, the proportions of direct exclusions was increased from 48% observed with the conventional starch technic to 76% with IEF.Keywords
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