Evidence for chromium acting as an essential trace element in insulin-dependent glucose uptake in cultured mouse myotubes

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Abstract
Previous work from this and other laboratories has suggested that the trace element chromium plays some role in glucose homeostasis. In this study, we sought to characterise an in vitro cell culture model in which the effects of chromium on insulin-dependent glucose uptake could be studied. Mouse C2C12 myoblasts were differentiated to form myotubes in culture in chromium-replete or chromium-poor media. Chromium levels in standard media were 0·56±0·01 μg/l (mean ± s.e.m.) compared with 0·09±0·01 μg/l in chromium-poor media. In chromium-poor media, insulin-stimulated uptake of radiolabelled glucose was reduced by almost 50% compared with that found in chromium-replete media. This decreased response could be restored by the addition of physiologically relevant (0·3 μg/l) concentrations of inorganic chromium (PPJournal of Endocrinology (1995) 144, 135–141

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