Galanin-mediated control of pain: enhanced role after nerve injury.
- 15 April 1992
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (8) , 3334-3337
- https://doi.org/10.1073/pnas.89.8.3334
Abstract
The endogenous inhibitory role of the neuropeptide galanin in pain transmission and spinal cord excitability was demonstrated by the use of a high-affinity galanin receptor antagonist, M-35 [galanin-(1-13)-bradykinin-(2-9)-amide]. M-35, which displaced 125I-labeled galanin from membranes of rat dorsal spinal cord with an IC50 of 0.3 nM, dose-dependently antagonized the effect of intrathecal galanin on the flexor reflex. M-35 potentiated the facilitation of the flexor reflex by conditioning stimulation of cutaneous unmyelinated afferents in rats with intact nerves and the potentiating effect of M-35 on the conditioning-stimulation-induced reflex facilitation of the cutaneous unmyelinated afferents was strongly enhanced after axotomy. These results demonstrate that endogenous galanin plays a tonic inhibitory role in the mediation of spinal cord excitability, and it is particularly noteworthy that this function of galanin is remarkably enhanced after peripheral nerve section.Keywords
This publication has 31 references indexed in Scilit:
- Further studies on galanin-, substance P-, and CGRP-like immunoreactivities in primary sensory neurons and spinal cord: Effects of dorsal rhizotomies and sciatic nerve lesionsExperimental Neurology, 1991
- Intrathecal injections of galanin and its antiserum affect nociceptive response of rat to mechanical, but not thermal, stimuliPain, 1991
- Galanin receptor and its ligands in the rat hippocampusEuropean Journal of Biochemistry, 1989
- Intrathecal galanin increases the latency in the tail‐flick and hot‐plate tests in mouseActa Physiologica Scandinavica, 1988
- Inhibitory effects of galanin on the isolated spinal cord of the newborn ratNeuroscience Letters, 1986
- Distribution of galaninlike immunoreactivity in the rat central nervous systemJournal of Comparative Neurology, 1986
- Vasoactive intestinal polypeptide (VIP) increases in the spinal cord after peripheral axotomy of the sciatic nerve originate from primary afferent neuronsBrain Research, 1986
- Galanin — a novel biologically active peptide from porcine intestineFEBS Letters, 1983
- Substance P: Depletion in the dorsal horn of rat spinal cord after section of the peripheral processes of primary sensory neuronsBrain Research, 1979
- Ongoing activity in peripheral nerves: The physiology and pharmacology of impulses originating from a neuroma☆Experimental Neurology, 1974