A complete set of repeat-depleted, PCR-amplifiable, human chromosome-specific painting probes
- 14 June 1999
- journal article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 84 (3-4) , 233-240
- https://doi.org/10.1159/000015266
Abstract
We describe the generation of a complete set of human chromosome-specific painting probes depleted in repetitive sequences. These probes yield highly specific signals when hybridized without the addition of a blocking agent, such as Cot-1 DNA, and without probe preannealing prior to hybridization. Fluorescent intensities and signal-to-background ratios for these probes are comparable to those of untreated probes hybridized with Cot-1 DNA. We demonstrate the suitability of these probes for applications with very complex probe sets, such as multiplex-FISH.Keywords
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